Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: Iron increases the susceptibility of colorectal cancer cells to compound Kushen Injection via the decrease of TOP2A and p53

doi: 10.1016/j.prmcm.2022.100058

Figure Lengend Snippet: Fig. 5. The expression levels of crucial proteins were obtained from network pharmacology analysis. ( A ) SW480 cells were treated with indicated drugs for 24, 48 and 72 h, and then analyzed with western blot assays for detecting the protein levels of p53. ( B ) The relative quantification of TOP2A and p53 was detected by western blot. The qualitative analysis was performed with Image Lab software. The data were displayed as the mean ± SD (n = 3).

Article Snippet: Anti-p53 (Cat#: 10,442–1-AP) nd anti-c-MYC antibodies (Cat#: 10,828–1-AP) were from Proteintech roup, Inc. Anti-PARP antibody (Cat#: 9542) was from Cell Signalng Technology (USA).

Techniques: Expressing, Western Blot, Software

Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: Iron increases the susceptibility of colorectal cancer cells to compound Kushen Injection via the decrease of TOP2A and p53

doi: 10.1016/j.prmcm.2022.100058

Figure Lengend Snippet: Fig. 6. Inhibition of TOP2A and p53 produced a synergistic antitumor efficiency in SW480 cells. ( A ) Effect of VP16 or SAHA on the protein levels of TOP2A or p53. Following VP16 or SAHA treatment for indicated times, SW480 cells were subjected to assays for detecting the protein levels of TOP2A or p53. ( B ) Effect of CKI and VP16 (a), FAC and SAHA(b), SAHA and VP16(c), FAC and pifithrin- 𝛼(d) combination on the cell growth of SW480 cells. SW480 cells were treated by indicated concentrations of drugs or their combinations, and subjected to in vitro antitumor assays. The data were presented as the mean ± SD ( n = 3).

Article Snippet: Anti-p53 (Cat#: 10,442–1-AP) nd anti-c-MYC antibodies (Cat#: 10,828–1-AP) were from Proteintech roup, Inc. Anti-PARP antibody (Cat#: 9542) was from Cell Signalng Technology (USA).

Techniques: Inhibition, Produced, In Vitro

Journal: STAR Protocols

Article Title: CyTOF mass cytometry analysis of human memory CD4 + T cells and memory B cells

doi: 10.1016/j.xpro.2022.101269

Figure Lengend Snippet: Surface marker antibody cocktail

Article Snippet: 146 Nd-conjugated anti-human IgD (clone IA6-2) (1:50 dilution) , Fluidigm , Cat#3146005B.

Techniques: Marker

Journal: STAR Protocols

Article Title: CyTOF mass cytometry analysis of human memory CD4 + T cells and memory B cells

doi: 10.1016/j.xpro.2022.101269

Figure Lengend Snippet:

Article Snippet: 146 Nd-conjugated anti-human IgD (clone IA6-2) (1:50 dilution) , Fluidigm , Cat#3146005B.

Techniques: Recombinant, Saline, Staining, Software, Cytometry